How soft is a single protein? The stress-strain curve of antibody pentamers with 5 pN and 50 pm resolutions.
نویسندگان
چکیده
Understanding the mechanical functionalities of complex biological systems requires the measurement of the mechanical compliance of their smallest components. Here, we develop a force microscopy method to quantify the softness of a single antibody pentamer by measuring the stress-strain curve with force and deformation resolutions, respectively, of 5 pN and 50 pm. The curve shows three distinctive regions. For ultrasmall compressive forces (5-75 pN), the protein's central region shows that the strain and stress are proportional (elastic regime). This region has an average Young's modulus of 2.5 MPa. For forces between 80 and 220 pN, the stress is roughly proportional to the strain with a Young's modulus of 9 MPa. Higher forces lead to irreversible deformations (plastic regime). Full elastic recovery could reach deformations amounting to 40% of the protein height. The existence of two different elastic regions is explained in terms of the structure of the antibody central region. The stress-strain curve explains the capability of the antibody to sustain multiple collisions without any loss of biological functionality.
منابع مشابه
A New Technique based on Strain Energy for Correction of Stress-strain Curve
Tensile stress-strain curve is of high importance in mechanics of materials particularly in numerical simulations of material deformations. The curve is usually obtained by experiment but is limited by necking phenomenon. Engineering stress-strain curve is converted to true stress-strain curve through simple formulas. The conversion, however, is correct up the point of necking. From this point ...
متن کاملConstruction of recombinant Pichia pastoris expressing single-chain antibody fragment against extracellular domain of EpCAM
Introduction: Epithelial cell adhesion molecule (EpCAM) is highly expressed on epithelial tumors. So, EpCAM is a valuable antigen for targeted therapy. Using monoclonal antibodies (mabs) is an attractive approach for targeted cancer therapy. Importantly, limitations of intact mabs including large size led to the development of antibody fragments such as single chain fragment variable (scfv). Pi...
متن کاملLine completion number of grid graph Pn × Pm
The concept of super line graph was introduced in the year 1995 by Bagga, Beineke and Varma. Given a graph with at least r edges, the super line graph of index r, Lr(G), has as its vertices the sets of r-edges of G, with two adjacent if there is an edge in one set adjacent to an edge in the other set. The line completion number lc(G) of a graph G is the least positive integer r for which Lr(G) ...
متن کاملCharacteristic Points of Stress-Strain Curve at High Temperature
Determination of critical points on hot stress-strain curve of metals is crucial in thermo-mechanical processes design. In this investigation a mathematical modeling is given to illustrate the behavior of metal during hot deformation processes such as hot rolling. The critical strain for the onset of dynamic recrystallization has been obtained as a function of strain at the maximum stress. In a...
متن کاملProduction, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen -1 of Enterotoxigenic Escherichia coli Causing Diarrhea
Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in both humans and animals. The contaminated food and water are the most common vehicles for ETEC infection. The colonization factor antigen (CFA-1) is a fimbriae protein that promotes adherence of the ETEC strain to the epithelium of the small intestine of the host. In this study IgY proteins were produced against the CFA-1 of ETEC in imm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Nanoscale
دوره 8 17 شماره
صفحات -
تاریخ انتشار 2016